FLOW CYTOMETRY

The INGM FACS Facility offers to the researchers an efficient and reliable personalized flow cytometry service.

Equipment

  • Two FACScantoII analyzers, equipped with 3 lasers (405, 488, and 633 nm) to analyze up to 10 parameters.
  • A BD FACSymphony A5 analyzer, equipped with 5 lasers (355, 405, 488, 561, 633) to analyze up to 30 parameters.
  • A FACSaria III SORP cell sorter, equipped with 5 lasers (355, 405, 488, 561 and 633 nm) to simultaneously analyze up to 20 parameters and to separate up to 4 cell subsets.
  • A FACSaria III cell sorter, equipped with 4 lasers (405, 488, 561, and 633 nm) to simultaneously analyze up to 14 parameters and to separate up to 4 cell subsets.
  • A Miltenyi Automacs Separator for high-speed magnetic cell sorting of multiple samples.

The major objectives of the facility are:

  • Provide cell sorting service for INGM researchers and external collaborators.
  • Perform sorting of samples from healthy donors or patients suffering from non-infectious diseases (inflammatory diseases, autoimmune diseases), cancer biopsies and organoids.
  • Set up, maintain and develop flow cytometry and cell sorting technology for basic and advanced research.
  • Directly provide sample acquisition and analysis in specific applications for INGM researchers and external collaborators.
  • Provide on-site training and assistance to prepare biomedical researchers to the use of optical instrumentation and to introduce to advanced flow cytometry applications.
  • Perform independent and collaborative research for the development of advanced flow cytometry protocols.
  • Perform collaborative research with INGM groups where highly specific flow cytometry and cell biology competences are required.

Our areas of expertise are:

  • Immuno-phenotyping and cell sorting of all immune cell subsets.
  • Immuno-phenotyping of hematopoietic stem cell subsets.
  • Immuno-phenotyping of mesenchymal stem cell.
  • Cell sorting of viable cytokine producing cells.
  • Multi-color extracellular and intracellular staining.
  • DNA content analysis (single color or multi-color).
  • Cell proliferation analysis.
  • Apoptosis analysis.
  • General cell sorting approaches for cell lines and transfected cells.

Activity

Our facility is equipped with two BD FACSCanto II analyzers, a BD FACSAria I cell sorter located in a BL3 room and dedicated to infected samples and a BD FACSaria III sorter dedicated to samples from healthy donors or patients suffering from non-infectious diseases (inflamatory diseases, autoimmune diseases, cancer ecc.).

Our areas of expertise are:

  • Immuno-phenotyping and cell sorting of all immune cell subsets.
  • Immuno-phenotyping of hematopoietic stem cell subsets.
  • Immuno-phenotyping of mesenchymal stem cell.
  • Cell sorting of viable cytokine producing cells.
  • Multi-color intracellular staining.
  • DNA content analysys (single color or multi-color).
  • Cell proliferation analisys.
  • Apoptosis.
  • General cell sorting approaches for cell lines and transfected cells.

Team

Nome / NameRuolo / RoleEmail
Mariacristina CrostiFacility Managercrosti@ingm.org
Marilena MancinoFacility Assistantmancino@ingm.org

Selected publications

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