FLOW CYTOMETRY

Our facility is equipped with BDSymphony A5 analyser, two BD FACSCanto II analyzers, a BD FACSAria III SORP cell sorter and a BD FACSaria III sorter dedicated to samples from healthy donors or patients suffering from non-infectious diseases (inflamatory diseases, autoimmune diseases, cancer ecc.) and TILs.
Activity
Our facility is equipped with one BDSymphony A5 analyser two BD FACSCanto II analyzers, two cell sorter BD FACSAria III SORP and BD FACSaria III sorter dedicated to samples from healthy donors or patients suffering from non-infectious diseases (inflamatory diseases, autoimmune diseases, cancer ecc.).

Equipment
–  2 FACScantoII analyzers, equipped with 3 lasers (405, 488, and 633 nm) to analyze up to 10 parameters.
–  1 BD FACSymphony A5 analyzer, equipped with 5 lasers (355, 405, 488, 561, 633) to analyze up to 30 parameters.
–  A FACSaria III SORP cell sorter, equipped with 5 lasers (355, 405, 488, 561 and 633 nm) to simultaneously analyze up to 20 parameters and to separate up to 4 cell subsets.
–  A FACSaria III cell sorter, equipped with 4 lasers (405, 488, 561, and 633 nm) to simultaneously analyze up to 14 parameters and to separate up to 4 cell subsets.
–  A Miltenyi Automacs Separator for high-speed magnetic cell sorting of multiple samples.

The major objectives of the facility are:

  • Set up, maintain and develop flow cytometry and cell sorting technology for basic and advanced research.
  • Provide cell sorting for INGM researchers and external collaborators.
  • Directly provide sample acquisition and analysis in specific applications for INGM researchers and external collaborators.
  • Provide on-site training and assistance to prepare biomedical researchers to the use of optical instrumentation and to introduce to advanced flow cytometry applications.
  • Perform independent and collaborative research for the development of advanced flow cytometry protocols.
  • Perform collaborative research with INGM groups where higly specific flow cytometry an cell biology competences are required.

Activity

Our facility is equipped with two BD FACSCanto II analyzers, a BD FACSAria I cell sorter located in a BL3 room and dedicated to infected samples and a BD FACSaria III sorter dedicated to samples from healthy donors or patients suffering from non-infectious diseases (inflamatory diseases, autoimmune diseases, cancer ecc.).

Our areas of expertise are:

  • Immuno-phenotyping and cell sorting of all immune cell subsets.
  • Immuno-phenotyping of hematopoietic stem cell subsets.
  • Immuno-phenotyping of mesenchymal stem cell.
  • Cell sorting of viable cytokine producing cells.
  • Multi-color intracellular staining.
  • DNA content analysys (single color or multi-color).
  • Cell proliferation analisys.
  • Apoptosis.
  • General cell sorting approaches for cell lines and transfected cells.

Team

Nome / NameRuolo / RoleEmail
Mariacristina CrostiFacility Managercrosti@ingm.org
Marilena MancinoFacility Assistantmancino@ingm.org

Equipment

• 2 FACScantoII analyzers, equipped with 3 lasers (405, 488, and 633 nm) to analyze up to 10 parameters.
• 1 BD FACSymphony A5 analyzer, equipped with 5 lasers (355, 405, 488, 561, 633) to analyze up to 30 parameters.
• A FACSaria III SORP cell sorter, equipped with 5 lasers (355, 405, 488, 561 and 633 nm) to simultaneously analyze up to 20 parameters and to separate up to 4 cell subsets.
• A FACSaria III cell sorter, equipped with 4 lasers (405, 488, 561, and 633 nm) to simultaneously analyze up to 14 parameters and to separate up to 4 cell subsets.
• A Miltenyi Automacs Separator for high-speed magnetic cell sorting of multiple samples..

Selected publications

INGM RESEARCH IS SUPPORTED BY