FLOW CYTOMETRY
The INGM FACS Facility offers to the researchers an efficient and reliable personalized flow cytometry service.
Equipment
- Two FACScantoII analyzers, equipped with 3 lasers (405, 488, and 633 nm) to analyze up to 10 parameters.
- A BD FACSymphony A5 analyzer, equipped with 5 lasers (355, 405, 488, 561, 633) to analyze up to 30 parameters.
- A FACSaria III SORP cell sorter, equipped with 5 lasers (355, 405, 488, 561 and 633 nm) to simultaneously analyze up to 20 parameters and to separate up to 4 cell subsets.
- A FACSaria III cell sorter, equipped with 4 lasers (405, 488, 561, and 633 nm) to simultaneously analyze up to 14 parameters and to separate up to 4 cell subsets.
- A new sorter MACSQuant® Tyto® , benchtop cell sorter equipped with 3 lasers, in BLS3 laboratory which allows for high speed, 10-parameter cell sorting.
- A Miltenyi Automacs Separator for high-speed magnetic cell sorting of multiple samples.
The major objectives of the facility are:
- Provide cell sorting service for INGM researchers and external collaborators.
- Perform sorting of samples from healthy donors or patients suffering from non-infectious diseases (inflammatory diseases, autoimmune diseases), cancer biopsies and organoids.
- Set up, maintain and develop flow cytometry and cell sorting technology for basic and advanced research.
- Directly provide sample acquisition and analysis in specific applications for INGM researchers and external collaborators.
- Provide on-site training and assistance to prepare biomedical researchers to the use of optical instrumentation and to introduce to advanced flow cytometry applications.
- Perform independent and collaborative research for the development of advanced flow cytometry protocols.
- Perform collaborative research with INGM groups where highly specific flow cytometry and cell biology competences are required.
Our areas of expertise are:
- Immuno-phenotyping and cell sorting of all immune cell subsets.
- Immuno-phenotyping of hematopoietic stem cell subsets.
- Immuno-phenotyping of mesenchymal stem cell.
- Cell sorting of viable cytokine producing cells.
- Multi-color extracellular and intracellular staining.
- DNA content analysis (single color or multi-color).
- Cell proliferation analysis.
- Apoptosis analysis.
- General cell sorting approaches for cell lines and transfected cells.
Activity
Our facility is equipped with two BD FACSCanto II analyzers, a BD FACSAria I cell sorter located in a BL3 room and dedicated to infected samples and a BD FACSaria III sorter dedicated to samples from healthy donors or patients suffering from non-infectious diseases (inflamatory diseases, autoimmune diseases, cancer ecc.).
Our areas of expertise are:
- Immuno-phenotyping and cell sorting of all immune cell subsets.
- Immuno-phenotyping of hematopoietic stem cell subsets.
- Immuno-phenotyping of mesenchymal stem cell.
- Cell sorting of viable cytokine producing cells.
- Multi-color intracellular staining.
- DNA content analysys (single color or multi-color).
- Cell proliferation analisys.
- Apoptosis.
- General cell sorting approaches for cell lines and transfected cells.
Team
| Nome / Name | Ruolo / Role | |
|---|---|---|
| Mariacristina Crosti | Facility Manager | crosti@ingm.org |
Selected publications
- Autosomal Dominant Hyper-IgE Syndrome Patients Retain IL10-Producing preTh17-Cells That Are Activated by Opportunistic Pathogens and Support IgE Production.
Moschetti G, Vasco C, Clemente F, Larghi P, Maioli S, Scarpa E, Carelli E, Pulvirenti N, Sarnicola ML, Crosti M, Bel Imam M, van de Veen W, Rizzello L, Abrignani S, Baselli LA, Dellepiane RM, Carrabba M, Fabio G, Geginat J.Allergy. 2026 Feb 25. - A human CAGinSTEM platform for decoding HTT repeats’ somatic instability links CAG interruption to HD pathology in neurons.
Zobel M, Damaggio G, Mignogna ML, Besusso D, Scalzo D, Cossu A, Trovesi C, Crosti M, Cortina F, Campus I, Formenti G, Mazzara S, Gregoretti F, Antonelli L, Oliva G, Zuccato C, Colonna V, Conforti P, Cereda M, Rossi RL, Maestri S, Scolz A, Iennaco R, Cattaneo E.Cell Rep. 2025 Dec 23;44(12):116685. - Generation of Human 3D Airway Assembloids for Advanced Modeling.
Iachini MC, Coglot A, Tace D, Elia N, Rusconi F, Cosentino F, Lopez G, Crosti M, Usal TD, Scarpa E, D’Amore A, Miceli V, Rosso L, Lazzari L. Int J Biol Sci. 2025 Oct 1;21(14):6234-6251 - Chromatin remodeling restrains oncogenic functions in prostate cancer.
Rosti V, Lembo G, Petrini C, Gorini F, Quadri R, Cordiglieri C, Mutarelli M, Salviato E, Casari E, Di Patrizio Soldateschi E, Montanari E, Albo G, Ripa F, Fasciani A, Crosti M, De Lorenzis E, Maggioni M, Vaira V, Vivo M, Ferrari F, Lanzuolo C.Nat Commun. 2025 Oct 16;16(1):9174 - Transplanted human striatal progenitors exhibit functional integration and modulate host circuitry in a Huntington’s disease animal model.
Scaramuzza L, Ribodino M, Cassarino C, Morrocchi M, Gomez Gonzalez GB, Parolisi R, Sozzi E, Turrini G, Cerrato V, Conforti P, Hoxha E, Tognato R, Galeotti G, Cordiglieri C, Crosti MC, Zucca S, Lorenzati M, Bovetti S, Spaiardi P, de’Sperati C, Biella G, Ottoboni L, Parmar M, Maestri S, Besusso D, Cattaneo E, Buffo A. Pharmacol Res. 2025 Sep;219:107905. - The Human Bone Marrow May Offer an IL-15-Dependent Survival Niche for EOMES+ Tr1-Like Cells.
Pulvirenti N, Vasco C, Righetti C, Dadova P, Boffa G, Laroni A, Vigo T, Raiola AM, Crosti MC, Maglie S, Valenti L, Prati D, Abrigani S, Uccelli A, Geginat J. Eur J Immunol. 2025 May;55(5) - V-ATPase in glioma stem cells: a novel metabolic vulnerability.
Storaci AM, Bertolini I, Martelli C, De Turris G, Mansour N, Crosti M, De Filippo MR, Ottobrini L, Valenti L, Polledri E, Fustinoni S, Caroli M, Fanizzi C, Bosari S, Ferrero S, Zadra G, Vaira V.
J Exp Clin Cancer Res. 2025 Jan 17;44(1):17. doi: 10.1186/s13046-025-03280-3.PMID: 39825382 - LINE1 modulate human T cell function by regulating protein synthesis during the life span
Burattin FV, Vadalà R, Panepuccia M, Ranzani V, Crosti M, Colombo FA, Ruberti C, Erba E, Prati D, Nittoli T, Montini G, Ronchi A, Pugni L, Mosca F, Ricciardi S, Abrignani S, Pietrasanta C, Marasca F, Bodega B . . Sci Adv. 2024 Oct 11;10(41):eado2134. doi: 10.1126/sciadv.ado2134. Epub 2024 Oct 9.PMID: 39383231 - An Intestinal Th17 Subset is Associated with Inflammation in Crohn’s Disease and Activated by Adherent-invasive Escherichia coli.
Paroni M, Leccese G, Ranzani V, Moschetti G, Chiara M, Perillo F, Ferri S, Clemente F, Noviello D, Conforti FS, Ferrero S, Karnani B, Bosotti R, Vasco C, Curti S, Crosti MC, Gruarin P, Rossetti G, Conte MP, Vecchi M, Pagani M, Landini P, Facciotti F, Abrignani S, Caprioli F, Geginat J. J Crohns Colitis. 2023 Dec 30;17(12):1988-2001. doi: 10.1093/ecco-jcc/jjad119.PMID: 37462681IF 8.7 - Fate mapping and scRNA sequencing reveal origin and diversity of lymph node stromal precursors.
Lenti E, Genovese L, Bianchessi S, Maurizio A, Sain SB, di Lillo A, Mattavelli G, Harel I, Bernassola F, Hehlgans T, Pfeffer K, Crosti M, Abrignani S, Evans SM, Sitia G, Guimarães-Camboa N, Russo V, van de Pavert SA, Garcia-Manteiga JM, Brendolan A. Immunity. 2022 Apr 12;55(4):606-622 - LINE1 are spliced in non-canonical transcript variants to regulate T cell quiescence and exhaustion.
Marasca F, Sinha S, Vadalà R, Polimeni B, Ranzani V, Paraboschi EM, Burattin FV, Ghilotti M, Crosti M, Negri ML, Campagnoli S, Notarbartolo S, Sartore-Bianchi A, Siena S, Prati D, Montini G, Viale G, Torre O, Harari S, Grifantini R, Soldà G, Biffo S, Abrignani S, Bodega B. Nat Genet. 2022 Feb;54(2):180-193
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