Human Genome Biology in health and disease 

In our lab we are interested in understanding the function of DNA repetitive elements in the epigenetic regulation of the transcriptional response of the cell. In particular, we are asking whether DNA repeats could influence the inter-individual variability between humans in terms of response to environmental cues, adaptation and predisposition to human diseases. DNA repeats cover almost 70% of the human genome, and their function was largely ignored for decades. Nevertheless, there are emerging evidences of the peculiar role of repeats in regulating the epigenome. For instance, DNA repeats are involved in chromosome structural organization, gene regulation, genome integrity, and evolution. Moreover, a plethora of potential functions for expressed repeat regions have been assayed using data generated by the ENCODE and FANTOM consortia. These studies indicate a functional role for repetitive DNA in cell identity by means of repeat- encoded, ncRNA-mediated mechanisms, acting both in cis and in trans. The work of our team may provide important mechanistic information and novel interpretation of how specific non coding DNA functions may directly influence cellular processes, such as differentiation and disease. Ultimate goal that to demonstrate that evolutionary acquisition of such elaborated fine modulation of transcription represent an added value for mammalians, and humans in particular, to react to environment, adapt to stress, and eventually predispose to better or worse outcomes in disease progression. Such an effort in elucidating DNA repeats molecular mechanisms might at the end get genome biology and epigenetics research closer to truly effective forms of personalized medicine.

Projects

  • Study of the transcriptional dynamics of LINE1 transposons in the identity and cellular plasticity of primary human CD4 + T lymphocytes circulating and infiltrating the tumor
  • Study of the epigenetic role of LINE1 elements and derived transcripts in the maintainance of human T-cell quiescence
  • Role of Transposable elements expression in colorectal cancer

Team

Nome / Name Ruolo / Role Email
Amanda Bianca Master Student bianca@ingm.org
Filippo Burattin PostDoc burattin@ingm.org
Marco Cominelli Master Student cominelli@ingm.org
Valeria Di Gioia PhD student digioia@ingm.org
Marco Gritti Master Student gritti@ingm.org
Simone Locatelli Master Student locatelli@ingm.org
Federica Marasca Research Scientist marasca@ingm.org
Maria Chiara Palma Predoctoral fellow palma@ingm.org
Francesco Panariello PostDoc panariello@ingm.org
Michele Panepuccia PhD Student
panepuccia@ingm.org
Benedetto Polimeni PostDoc polimeni@ingm.org
Valeria Ranzani Research Scientist, Bioinformatician ranzani@ingm.org
Marta Sacco Master Student sacco@ingm.org
Giulia Solbiati Predoctoral Fellow solbiati@ingm.org
Alen Stambolliu PhD Student stambolliu@ingm.org
Rebecca Vadalà PostDoc vadala@ingm.org
Jan Zamporlini Fellow zamporlini@ingm.org

Publications

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