MICROSCOPIA
La facility ospita una vasta gamma di microscopi ottici a fluorescenza, set-ups per live-imaging e sistemi di microscopia avanzata confocale, super-risoluzione e High-Content. Forniamo training, supervisione e assistenza agli utenti, dalla preparazione del campione, al processamento dell’immagine e all’analisi quantitativa del dato, sviluppando e coordinando progetti collaborativi in ogni ramo della ricerca biologica.
Attività
- Corsi di microscopia base e avanzata, incluse la microscopia a super-risoluzione, la microscopia confocale e l’imaging in High-Content.
- Acquisizioni multi-colore e 3D rendering in microscopia confocale e super-risoluzione.
- Acquisizioni multidimensionali (4D/5D) in condizioni vitali a lunga durata (fino a diversi giorni).
- Acquisizioni live in veloci dinamiche (flussi di calcio, trasportoo mitocondriale, etc.) ad alta risoluzione.
- Sviluppo di specifiche MACRO, su richiesta dell’utente, per procedure di acquisizione automatizzata e di pipeline per automatizzare l’analisi dei dati di microscopia.
- Servizio di supporto e assistenza per la messa appunto di marcature in fluorescenza, tecniche di detezione del segnale e interpretazione del dato in fluorescenza.
- Servizio di analisi dell’immagine e corsi specifici all’analisi di immagine in microscopia.
Team
| Nome / Name | Ruolo / Role | |
|---|---|---|
| Chiara Cordiglieri | Facility Manager | cordiglieri@ingm.org |
Strumenti
- Microscopio Stedycon per microscopia STED a super risoluzione e microscopia confocale.
- Microscopio confocale a scansione laser Leica SP5 con camera ambientale motorizzata per acquisizioni automatizzate.
- Microscopio Video-Confocal-SuperResolution Nikon TiE/CREST multifunzione a doppia eccitazione Laser e LED con camera ambientale e acquisizione multicanale (fino a 24 colori), con detezione confocale in spinning disk e in luce strutturata per super-risoluzione.
- Microscopio invertito Leica ad ampio spettro con camera ambientale motorizzata.
- Diversi microscopi a epifluorescenza per esperimenti di routine.
- Workstation di analisi d’immagine equipaggiate di software ad hoc: NIS-Elements AR, Las-X, Fiji-ImageJ, Cell Profiler, iLastik, qPath, Volocity.
Pubblicazioni
- GMMA decorated with mucin 1 Tn/STn mimetics elicit specific antibodies response and inhibit tumor growth.
Pesce E, Sodini A, Palmieri E, Valensin S, Tinti C, Rossi M, De Rosa A, Fragai M, Papi F, Cordiglieri C, Berti F, Grifantini R, Micoli F, Nativi C.
NPJ Vaccines. 2025 Apr 15;10(1):71. - Macrophages producing chondroitin sulfate proteoglycan-4 induce neuro-cardiac junction impairment in Duchenne muscular dystrophy.
Milan M, Maiullari F, Chirivì M, Ceraolo MG, Zigiotto R, Soluri A, Maiullari S, Landoni E, Silvestre DD, Brambilla F, Mauri P, De Paolis V, Fratini N, Crosti MC, Cordiglieri C, Parisi C, Calogero A, Seliktar D, Torrente Y, Lanzuolo C, Dotti G, Toccafondi M, Bombaci M, De Falco E, Bearzi C, Rizzi R.
J Pathol. 2025 Jan;265(1):1-13. - A millifluidic bioreactor allows the long term culture of primary lymphocytes or CD34+ hematopoietic cells while allowing the detection of tumorigenic expansion.
Ritter P, Oliveto S, Cordiglieri C, Fasciani A, Di Buduo CA, Della Volpe L, Bocconi A, Conci C, Miguel CP, Di Micco R, Balduini A, Raimondi MT, Biffo S.
Front Bioeng Biotechnol. 2024 Oct 2;12:1388312. - Testing calpain inhibition in tumor endothelial cells: novel targetable biomarkers against glioblastoma malignancy.
Guarnaccia L, Navone SE, Begani L, Barilla E, Garzia E, Campanella R, Miozzo M, Fontana L, Alotta G, Cordiglieri C, Gaudino C, Schisano L, Ampollini A, Riboni L, Locatelli M, Marfia G.
Front Oncol. 2024 Aug 2;14:1355202. - Neuroprotection by ADAM10 inhibition requires TrkB signaling in the Huntington’s disease hippocampus.
Scolz A, Vezzoli E, Villa M, Talpo F, Cazzola J, Raffin F, Cordiglieri C, Falqui A, Pepe G, Maglione V, Besusso D, Biella G, Zuccato C.
Cell Mol Life Sci. 2024 Aug 7;81(1):333. - Huntington’s disease cellular phenotypes are rescued non-cell autonomously by healthy cells in mosaic telencephalic organoids.
Galimberti M, Nucera MR, Bocchi VD, Conforti P, Vezzoli E, Cereda M, Maffezzini C, Iennaco R, Scolz A, Falqui A, Cordiglieri C, Cremona M, Espuny-Camacho I, Faedo A, Felsenfeld DP, Vogt TF, Ranzani V, Zuccato C, Besusso D, Cattaneo E.
Nat Commun. 2024 Aug 2;15(1):6534. - The Impact of 3D Nichoids and Matrix Stiffness on Primary Malignant Mesothelioma Cells.
Oliveto S, Ritter P, Deroma G, Miluzio A, Cordiglieri C, Benvenuti MR, Mutti L, Raimondi MT, Biffo S.
Genes (Basel). 2024 Feb 1;15(2):199. - Combined RNA interference and gene replacement therapy targeting MFN2 as proof of principle for the treatment of Charcot-Marie-Tooth type 2A.
Rizzo F, Bono S, Ruepp MD, Salani S, Ottoboni L, Abati E, Melzi V, Cordiglieri C, Pagliarani S, De Gioia R, Anastasia A, Taiana M, Garbellini M, Lodato S, Kunderfranco P, Cazzato D, Cartelli D, Lonati C, Bresolin N, Comi G, Nizzardo M, Corti S.
Cell Mol Life Sci. 2023 Nov 25;80(12):373. - TMEM123 a key player in immune surveillance of colorectal cancer.
Pesce E, Cordiglieri C, Bombaci M, Eppenberger-Castori S, Oliveto S, Manara C, Crosti M, Ercan C, Coto M, Gobbini A, Campagnoli S, Donnarumma T, Martinelli M, Bevilacqua V, De Camilli E, Gruarin P, Sarnicola ML, Cassinotti E, Baldari L, Viale G, Biffo S, Abrignani S, Terracciano LM, Grifantini R.
Front Immunol. 2023 Jun 23;14:1194087. - The Role of Adhesion Molecules and Extracellular Vesicles in an In Vitro Model of the Blood-Brain Barrier for Metastatic Disease.
Vasco C, Rizzo A, Cordiglieri C, Corsini E, Maderna E, Ciusani E, Salmaggi A.
Cancers (Basel). 2023 Jun 3;15(11):3045. - Long-term retention of gold nanoparticles in the liver is not affected by their physicochemical characteristics.
Fernandez Alarcon J, Soliman M, Lüdtke TU, Clemente E, Dobricic M, Violatto MB, Corbelli A, Fiordaliso F, Cordiglieri C, Talamini L, Sitia G, Moya S, Bigini P, Monopoli MP.
Nanoscale. 2023 May 18;15(19):8740-8753. doi: 10.1039/d3nr00685a. PMID: 37097471. - In vitro-derived medium spiny neurons recapitulate human striatal development and complexity at single-cell resolution.
Conforti P, Bocchi VD, Campus I, Scaramuzza L, Galimberti M, Lischetti T, Talpo F, Pedrazzoli M, Murgia A, Ferrari I, Cordiglieri C, Fasciani A, Arenas E, Felsenfeld D, Biella G, Besusso D, Cattaneo E.
Cell Rep Methods. 2022 Dec 19;2(12):100367. - Mapping of functional SARS-CoV-2 receptors in human lungs establishes differences in variant binding and SLC1A5 as a viral entry modulator of hACE2.
Miluzio A, Cuomo A, Cordiglieri C, Donnici L, Pesce E, Bombaci M, Conti M, Fasciani A, Terracciano L, Manganaro L, Toccafondi M, Scagliola A, Oliveto S, Ricciardi S, Grifantini R, De Francesco R, Abrignani S, Manfrini N, Biffo S.
EBioMedicine. 2023 Jan;87:104390. - Synthesis and Assessment of the In Vitro and Ex Vivo Activity of Salicylate Synthase (Mbti) Inhibitors as New Candidates for the Treatment of Mycobacterial Infections.
Mori M, Stelitano G, Griego A, Chiarelli LR, Cazzaniga G, Gelain A, Pini E, Camera M, Canzano P, Fumagalli A, Scarpa E, Cordiglieri C, Rizzello L, Villa S, Meneghetti F.
Pharmaceuticals (Basel). 2022 Aug 11;15(8):992. - Unusual Association of NF-κB Components in Tumor-Associated Macrophages (TAMs) Promotes HSPG2-Mediated Immune-Escaping Mechanism in Breast Cancer.
De Paolis V, Maiullari F, Chirivì M, Milan M, Cordiglieri C, Pagano, La Manna AR, De Falco E, Bearzi C, Rizzi R, Parisi C.
Int J Mol Sci. 2022 Jul 18;23(14):7902. - Heterogeneity of Latency Establishment in the Different Human CD4+ T Cell Subsets Stimulated with IL-15.
Butta GM, Bozzi G, Gallo G, Copaloni G, Cordiglieri C, Crosti M, Mancino M, Prati D, Simon V, Gori A, Bandera A, De Francesco R, Manganaro L.
J Virol. 2022 May 25;96(10):e0037922. - Exosomes Recovered From the Plasma of COVID-19 Patients Expose SARS-CoV-2 Spike-Derived Fragments and Contribute to the Adaptive Immune Response.
Pesce E, Manfrini N, Cordiglieri C, Santi S, Bandera A, Gobbini A, Gruarin P, Favalli A, Bombaci M, Cuomo A, Collino F, Cricrì G, Ungaro R, Lombardi A, Mangioni D, Muscatello A, Aliberti S, Blasi F, Gori A, Abrignani S, De Francesco R, Biffo S, Grifantini R.
Front Immunol. 2022 Jan 17;12:785941. - The dominant p.Thr274Pro mutation in the von Willebrand factor propeptide causes the von Willebrand disease type 1 phenotype in two unrelated patients.
Pagliari MT, Baronciani L, Cordiglieri C, Colpani P, Cozzi G, Siboni SM, Peyvandi F.
Haemophilia. 2022 Mar;28(2):292-300. - Targeting of eIF6-driven translation induces a metabolic rewiring that reduces NAFLD and the consequent evolution to hepatocellular carcinoma.
Scagliola A, Miluzio A, Ventura G, Oliveto S, Cordiglieri C, Manfrini N, Cirino D, Ricciardi S, Valenti L, Baselli G, D’Ambrosio R, Maggioni M, Brina D, Bresciani A, Biffo S.
Nat Commun. 2021 Aug 12;12(1):4878. - Tumor Extracellular Matrix Stiffness Promptly Modulates the Phenotype and Gene Expression of Infiltrating T Lymphocytes.
Chirivì M, Maiullari F, Milan M, Presutti D, Cordiglieri C, Crosti M, Sarnicola ML, Soluri A, Volpi M, Święszkowski W, Prati D, Rizzi M, Costantini M, Seliktar D, Parisi C, Bearzi C, Rizzi R.
Int J Mol Sci. 2021 May 30;22(11):5862. - Clonally expanded EOMES+ Tr1-like cells in primary and metastatic tumors are associated with disease progression.
Bonnal RJP, Rossetti G, Lugli E, De Simone M, Gruarin P, Brummelman J, Drufuca L, Passaro M, Bason R, Gervasoni F, Della Chiara G, D’Oria C, Martinovic M, Curti S, Ranzani V, Cordiglieri C, Alvisi G, Mazza EMC, Oliveto S, Silvestri Y, Carelli E, Mazzara S, Bosotti R, Sarnicola ML, Godano C, Bevilacqua V, Lorenzo M, Siena S, Bonoldi E, Sartore-Bianchi A, Amatu A, Veronesi G, Novellis P, Alloisio M, Giani A, Zucchini N, Opocher E, Ceretti AP, Mariani N, Biffo S, Prati D, Bardelli A, Geginat J, Lanzavecchia A, Abrignani S, Pagani M.
Nat Immunol. 2021 Jun;22(6):735-745. - Development of a Specific Monoclonal Antibody to Detect Male Cells Expressing the RPS4Y1 Protein
Spena S, Cordiglieri C, Garagiola I, Peyvandi F.
Int J Mol Sci. 2021 Feb 18;22(4):2001. doi: 10.3390/ijms22042001. - SREBP2 delivery to striatal astrocytes normalizes transcription of cholesterol biosynthesis genes and ameliorates pathological features in Huntington’s disease
Birolini G, Verlengia G Talpo F, Maniezzi C, Zentilin L, Giacca M, Conforti P, Cordiglieri C, Caccia C, Leoni V.
BRAIN-2020-02219.R1 - Frataxin gene editing rescues Friedreich’s ataxia pathology in dorsal root ganglia organoid-derived sensory neurons
Mazzara PG, Muggeo S, Luoni M, Massimino L, Zaghi M, Tajalli-Tehrani Valverde P, Brusco S, Marzi MJ, Palma C, Colasante G, Iannielli A, Paulis M, Cordiglieri C, Giannelli SG, Podini P, Gellera C, Taroni F, Nicassio F, Rasponi M, Broccoli V
Nat Commun. 2020 Aug 21;11(1):4178. doi:10.1038/s41467-020-17954-3 - Evidence for a pathogenic role of extrafollicular, IL-10–producing CCR6+B helper T cells in systemic lupus erythematosus
Facciotti F, Larghi P, Bosotti R, Vasco C, Gagliani N, Cordiglieri C, Mazzara S, Ranzani V, Rottoli E, Curti S, Penatti A, Karnani B, Kobayashi Y, Crosti M, Bombaci M, van Hamburg JP, Rossetti G, Gualtierotti R, Gerosa M, Gatti S, Torretta S, Pignataro L, Tas SW, Abrignani S, Pagani M, Grassi F, Meroni PL, Flavell RA, and Geginat J.
PNAS, March 17,2020; http://doi. org/10.1073/pnas.1917834117. - Tumor-Educated Platelets and Angiogenesis in Glioblastoma: Another Brick in the Wall for Novel Prognostic and Targetable Biomarkers, Changing the Vision from a Localized Tumor to a Systemic Pathology
Campanella R, Guarnaccia L, Cordiglieri C, Trombetta E, Caroli M, Carrabba G, La Verde N, Rampini P, Gaudino C, Costa A, Luzzi S, Mantovani G, Locatelli M, Riboni L, Navone SE and Marfia G.
Cells 2020, 9, 294; doi:10.3390/cells9020294. Published: 25 January 2020 - Reconstitution of the Human Nigro-striatal Pathway on-a-Chip Reveals OPA1-Dependent Mitochondrial Defects and Loss of Dopaminergic Synapses
Iannielli A, Ugolini GS, Cordiglieri C, et al.
Cell Rep. 2019;29(13):4646–4656.e4. - Novel biomarkers for Primary Biliary Cholangitis to improve diagnosis and understand underlying regulatory mechanisms.
Bombaci M, Pesce E, Torri A, Carpi D, Crosti M, Lanzafame M, Cordiglieri C, Sinisi A, Moro M, Bernuzzi F, Gerussi A, Geginat J, Muratori L, Terracciano LM, Invernizzi P, Abrignani S, Grifantini R.
Liver Int. 2019 Nov;39(11):2124-2135. - Aspirin Affects Tumor Angiogenesis and Sensitizes Human Glioblastoma Endothelial Cells to Temozolomide, Bevacizumab, and Sunitinib, Impairing Vascular Endothelial Growth Factor-Related Signaling.
Navone SE1 Guarnaccia L, Cordiglieri C, Crisà FM, Caroli M, Locatelli M, Schisano L, Rampini P, Miozzo M, La Verde N, Riboni L, Campanella R, Marfia G.
World Neurosurg. (2018); Aug 23. pii: S1878-8750(18)31863-1. - Small Molecule Antagonists of NAADP-Induced Ca2+ Release in T-Lymphocytes Suggest Potential Therapeutic Agents for Autoimmune Disease.
Zhang B, Watt JM, Cordiglieri C, Dammermann W, Mahon MF, Flügel A, Guse AH, Potter BVL
Sci Rep. (2018); 13;8(1):16775. - Angiogenesis in human brain tumors: screening of drug response through a patient-specific cell platform for personalized therapy.
Guarnaccia L, Navone SE, Trombetta E, Cordiglieri C, Cherubini A, Crisà FM, Rampini P, Miozzo M, Fontana L, Caroli M, Locatelli M, Riboni L, Campanella R, Marfia G
Sci Rep. (2018); 8(1):8748. - Mechanical loading of intervertebral disc modulates microglia proliferation, activation, and chemotaxis.
Navone SE, Peroglio M, Guarnaccia L, Beretta M, Grad S, Paroni M, Cordiglieri C, Locatelli M, Pluderi M, Rampini P, Campanella R, Alini M, Marfia G
Osteoarthritis Cartilage. (2018) May 1. pii: S1063-4584(18)31220-2 - TCTN2: a novel tumor marker with oncogenic properties.
Cano-Rodriguez D, Campagnoli S, Grandi A, Parri M, Camilli E, Song C, Jin B, Lacombe A, Pierleoni A, Bombaci M, Cordiglieri C, Ruiters MH, Viale G, Terracciano L, Sarmientos P, Abrignani S, Grandi G, Pileri P, Rots MG, Grifantini R.
Oncotarget. (2017) 24;8 (56):95256-95269 - Platelets from glioblastoma patients promote angiogenesis of tumor endothelial cells and exhibit increased VEGF content and release.
Di Vito C, Navone SE, Marfia G, Abdel Hadi L, Mancuso ME, Pecci A, Crisa FM, Berno V, Rampini P, Campanella R, Riboni L.
Platelets (2017) 28:585-594
- Uncontrolled IL-17 Production by Intraepithelial Lymphocytes in a Case of non-IPEX Autoimmune Enteropathy.
Paroni M, Magarotto A, Tartari S, Nizzoli G, Larghi P, Ercoli G, Gianelli U, Pagani M, Elli L, Abrignani S, Conte D, Geginat J, Caprioli F.
Clin Transl Gastroenterol (2016) 7:e182
- A Myc-driven self-reinforcing regulatory network maintains mouse embryonic stem cell identity.
Fagnocchi L, Cherubini A, Hatsuda H, Fasciani A, Mazzoleni S, Poli V, Berno V, Rossi RL, Reinbold R, Endele M, Schroeder T, Rocchigiani M, Szkarlat Z, Oliviero S, Dalton S, Zippo A.
Nat Commun (2016) 7:11903
- The Adipose Mesenchymal Stem Cell Secretome Inhibits Inflammatory Responses of Microglia: Evidence for an Involvement of Sphingosine-1-Phosphate Signalling.
Marfia G, Navone SE, Hadi LA, Paroni M, Berno V, Beretta M, Gualtierotti R, Ingegnoli F, Levi V, Miozzo M, Geginat J, Fassina L, Rampini P, Tremolada C, Riboni L, Campanella R.
Stem Cells Dev (2016) 25:1095-107
- The vacuolar H+ ATPase is a novel therapeutic target for glioblastoma.
Di Cristofori A, Ferrero S, Bertolini I, Gaudioso G, Russo MV, Berno V, Vanini M, Locatelli M, Zavanone M, Rampini P, Vaccari T, Caroli M, Vaira V.
Oncotarget (2015) 6:17514-31
- NS5A inhibitors impair NS5A-phosphatidylinositol 4-kinase IIIα complex formation and cause a decrease of phosphatidylinositol 4-phosphate and cholesterol levels in hepatitis C virus-associated membranes.
Reghellin V, Donnici L, Fenu S, Berno V, Calabrese V, Pagani M, Abrignani S, Peri F, De Francesco R, Neddermann P.
Antimicrob Agents Chemother (2014) 58:7128-40
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